Bd facsaria ii safety and limitations manual

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The site you are about to visit is operated by a third party. The link to this site neither makes nor implies any representation or warranty for any products or services offered on a third-party site and is intended only to enable convenient access to the third-party site and for no other purpose. Do you want to continue? Cancel Yes. Old Browser. For the best web browsing experience, please use Chrome, Safari or Firefox, minimum versions Request Quote. The flow cytometer has been verified to meet personnel and product protection standards for a Class II Type A2 biosafety cabinet and to meet many local standards Precisely integrated fluidic and optical systems maximize signal detection Optimized fiber-launched lasers improve sensitivity and resolution for each color in a multicolor assay The system can be expanded with additional lasers via a field upgrade.

Complete cell surface phenotype of every cell sorted into a multiposition sort device, such as a well tray Sort and tray position information available on an event-by-event basis according to the X and Y coordinates of the sort collection device Ability to precisely trace back post-sorting results to the flow characteristics of the specific cell.

BD and Baker-designed cabinet meets biosafety standards and saves space The Biosafety Cabinet BSC controls the direction, volume and speed of airflow to direct potentially harmful particles away from the operator.

Since the flow cytometer and the BSC were designed for a seamless fit, a BSC can be added later in a field upgrade that takes less than a day to install The BSC protects the operator from aerosol exposure during a sort and the built-in and independently operated Aerosol Management System AMS also evacuates aerosols The enclosed sample-to-sort pathway, the Class II Type A2 biosafety cabinet and the Aerosol Management System make aerosol management fully redundant Since these systems are independent, they can be tested individually to ensure optimal operation and backup protection.

Sensitivity to resolve dim staining populations The lasers are specifically selected for optimal signal-to-noise separation and improve the resolution of dim populations, thereby enabling more effective gating for sorting. Integrated Nozzle FAQ. Contact Us. Sapphire is a trademark and Coherent is a registered trademark of Coherent, Inc. Spherotech, Inc.

All other company and product names might be trademarks of the respective companies with which they are associated. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.

This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his or her own expense.

Technical Assistance. Laser Safety. Laser Product Classification. Precautions for Safe Operation. Electrical Safety. Biological Safety. General Safety. Precaution Labels. Fluidics Cart. Containers and Connectors. Power and Operation. Flow Cytometer. Fluidics Components. Optics System.

Instrument Electronics. Fluid Movement. Sheath Flow. Sample Flow. Signal Generation. Light Scatter. Fluorescent Signal. Signal Detection. Detector Arrays. Electronic Processing. Pulse Parameters. Laser Delay. Drop Formation. Side Stream Formation. Drop Charging.

Conflict Resolution During Sorting. Workspace Components. Instrument Controls. Fluidics Controls. Fluidics Level Indicators. Instrument Configuration. Instrument Status Report. Acquisition Controls. Sorting Controls. Sort Menu. Sort Setup. Sort Layout. Sort Report. Instrument Startup. Setting Up the Breakoff.

Setting Up the Fluidics Cart. Instrument Quality Control. Setting Up the QC Experiment. Running QC Particles. Tracking QC Results. Reusing the Experiment.

Optimization of Instrument Settings. Creating the Experiment. Adjusting the Voltages and Threshold. Adjusting Area Scaling. Calculating Compensation. Data Recording and Analysis. Setting Up the Experiment. Recording Data. Analyzing Data. Saving the Analysis. Sample QC Log. Setting Up for Sorting. Daily Shutdown. Fluidics Shutdown. External Cleaning. Scheduled Maintenance. Internal Cleaning. Purging Filters. Changing Fluid Filters. Changing the Sample Lines.

Changing Air Filters. Changing the Bubble Filter. Unscheduled Maintenance. Changing the Nozzle. Adjusting the Nozzle Orifice. Cleaning a Nozzle. Cleaning the Camera Windows. Troubleshooting the Stream. Troubleshooting the Breakoff. Sorting Troubleshooting. Acquisition Troubleshooting.

Fluidics Troubleshooting. Electronics Troubleshooting. Instrument Supplies. Optical Components. Accessory Kit. Other Replacement Parts. Laser Specifications. Instrument Setup Particles. Instrument Options. Application Options. Two-Laser System. Three-Laser System. Defining a Custom Configuration. If you are not familiar with the Windows operating system, refer to the documentation provided with your computer. Instructions for routine acquisition, analysis, and sorting can be found in Chapters 4 and 5.

The following tables list conventions used throughout this guide. Table 1 lists the symbols that are used in this guide or on safety labels to alert you to a potential hazard. Text and keyboard conventions are shown in Table 2. Caution: hazard or unsafe practice that could result in material damage, data loss, minor or severe injury, or death. If additional assistance is required, contact your local BD Biosciences technical support representative or supplier. For instrument support from within the US, call For support from within Canada, call Do not perform instrument maintenance or service except as specifically stated.

Keep this safety information available for reference. Lasers or laser systems emit intense, coherent electromagnetic radiation that has the potential of causing irreparable damage to human skin and eyes. The main hazard of laser radiation is direct or indirect exposure of the eye to thermal radiation from the visible and near-infrared spectral regions —1, nm.

Direct eye contact can cause corneal burns, retinal burns, or both, and possible blindness. There are other potentially serious hazards in other spectral regions. For cytometers that contain UV lasers, excessive ultraviolet exposure produces an intolerance to light photophobia accompanied by redness, a tearing discharge from the mucous membrane lining the inner surface of the eyelid conjunctiva , shedding of the corneal cell layer surface exfoliation , and stromal haze.

These effects can be the result of laser exposure lasting only a fraction of a second. Laser hazard levels depend on laser energy content and the wavelengths used. A numbered system is used to categorize laser products according to different hazard levels.

The higher the classification number, the greater the potential hazard. The lasers and the laser energy are fully contained within the instrument structure and call for no special work area safety requirements except during service procedures. These procedures are to be carried out only by BD Biosciences service personnel. Modification or removal of the optics covers or laser shielding could result in exposure to hazardous laser radiation.

To prevent irreparable damage to human skin and eyes, do not remove the optics covers or laser shielding, adjust controls, or attempt to service the instrument any place where laser warning labels are attached see Precaution Labels on page xviii. Keep all instrument doors closed during instrument operation. When operated under these conditions, the instrument poses no danger of exposure to hazardous laser radiation.

Lethal electrical hazards are present in some lasers, particularly in laser power supplies. Many portions of the electrical system, including the printed circuit boards, are at a dangerous voltage level.

To prevent shock injury, follow these guidelines. Do not use extension cords. If cords, plugs, or cables are damaged, immediately contact BD Biosciences for a replacement. Have a qualified electrician replace any ungrounded receptacles with properly grounded receptacles in accordance with the local electrical code. Contact your local BD representative for more information. Do not plug the fluidics cart power cord into a wall outlet.

Plug the cord into the cytometer only. This ensures proper electrical grounding and protects against electrical shock or damage to the instrument. A 12,volt potential exists between the deflection plates when they are on. Contact with the charged plates results in serious electrical shock. Do not touch the deflection plates when the voltage warning light is illuminated, or when the software indicates that the plate voltage is on. The plates remain energized even when the sort block door is opened.

The barb and the stream-charging wire conduct electrical charge during sorting. To prevent shock, do not touch the barb or the stream-charging wire when the voltage warning light is illuminated or any time during sorting.

All biological specimens and materials coming into contact with them can transmit potentially fatal disease. To prevent exposure to biohazardous agents, follow these guidelines. Dispose of waste using proper precautions and in accordance with local regulations. Never pipette by mouth. Wear suitable protective clothing, eyewear, and gloves. Dispose of waste in accordance with local regulations.

Use proper precaution and wear suitable protective clothing, eyewear, and gloves. Always disconnect the tank from the wet cart before you empty it. Wait at least 1 minute for pressure to dissipate before you remove the waste cap or sensor. Failure to do so could cause the waste tank to malfunction.

For tracking, write the date on the waste cap label each time you change the cap. If wet, the filter in the cap will cause the tank to malfunction. To keep the cap dry, place it on the bench label-. When acquiring biohazardous samples, follow universal precautions at all times. Keep the sort block door closed during sorting. If you need to access the sort block, turn off the stream before opening the door.

For information on laboratory safety, refer to the following guidelines. NCCLS documents can be ordered online at www. Biosafety guidelines for sorting of unfixed cells. Movement of mechanical parts within the instrument can pinch or injure your hands or fingers. To prevent injury by moving parts, follow these precautions. Do not place objects underneath the sample injection chamber. The drawer can close at any time without warning if a clog is detected during sorting.

Do not place objects inside the sort collection chamber, and do not attempt to. The microtiter tray stage can move suddenly during the sorting process. Sorting continues even when the sort collection chamber door is open. Do not remove these labels. Use appropriate precautions to avoid injury by the indicated hazard.

See the previous sections for more information. Consult the user's guide for instructions. Peel Here. NOTE: Voltage is set by your service engineer during installation. This instrument is for Research Use Only.

Not for use in diagnostic or therapeutic procedures. BD Biosciences delivers software and workstations that are intended for running the instruments supplied by BD Biosciences.

BD Biosciences does not make any warranty with respect to the workstation remaining virus free after installation. The instrument can be operated at varied pressures and can acquire up to 70, events per second. With its fixed-optics design and digital electronics, the BD FACSAria flow cytometer enables multicolor analysis of up to 13 fluorescent markers and two scatter parameters at a time. For technical information about how the instrument works, see Chapter 2.

A separate fluidics cart supplies sheath and cleaning fluids and collects waste from the instrument Figure The self-contained fluidics cart supplies the required air pressure and vacuum, which eliminates the need for an external source although the cart can be hooked up to an in-house air source, if one is available.

The air pumps provide pressure from 2 to 75 psi to accommodate a variety of cell sorting applications. Figure Fluidics cart. The fluidics cart holds four L containers two sheath and two waste , and three 5-L auxiliary cleaning fluid containers Figure Figure Fluidics cart containers. To prevent foaming, do not fill the containers with solutions containing a high concentration of detergent.

The fluidics cart connects directly to the flow cytometer unit via a power cord, fluid hoses, serial communication cable, and air line Figure on page Receptacles for the aerosol management and temperature control options are also located within the connection panel.

The position of the fluidics cart is constrained only by the length of the connecting cables and hoses, which extend up to 9 feet 2.

Typically, the cart is placed to the left or underneath the cytometer. Figure Fluidics cart power and fluid line connectors. To connect the fluidics cart to an external air source, open the shutter above the Communication port, switch on the Auxiliary Air Supply, and attach the external air line to the air input connector.

The external air supply should provide 80— psi. If your laboratory runs the cytometer continuously and does not shut down at the end of the day, switch on the Auxiliary Air Supply for 15 seconds every 8 hours every 4 hours in an extremely humid climate. Doing this empties the cart condensation trap and prevents excess moisture from overflowing the trap or causing cart damage. Power to the fluidics cart is supplied and controlled through the flow cytometer.

The fluidics cart voltage settings have been configured to match the supply voltage by your service engineer. To properly operate the fluidics cart, plug the fluidics cart power cord only into the power receptacle on the instrument Figure on page Do not plug the power cord directly into a wall socket.

Do not change the input voltage selection jumpers on the fluidics cart fuse holder. When the stream is on, air pressure fluctuates between 80— psi Figure A pressure reading of less than 80 psi or greater than psi indicates that the fluidics cart is not functioning properly. If this occurs, contact your BD Biosciences service representative for assistance.

Do not operate the instrument outside the normal air pressure range. Figure Fluidics cart flow gauge. The benchtop flow cytometer contains the major components for all three subsystems fluidics, optics, and electronics. The instrument can be set up on a typical laboratory benchtop or table, and it requires only a amp electrical outlet. No special facilities are required. To view the fluidics components, open the side door and lift the flow cell access door; to view the optics components, open the optics access door.

The power panel and connectors are found on the left side of the instrument. Other electronic components are embedded within the instrument and do not need user adjustment. The flow cell access door is equipped with a shutter mechanism that shuts off the laser light when the door is opened. To ensure there is no interruption to data acquisition, do not open the door while sorting or recording.

When the fluidics system is activated, sheath fluid is pumped from the fluidics cart into a plenum reservoir where the fluidics system is pressurized. From the reservoir, fluid is pumped into the cuvette flow cell where hydrodynamic focusing forces particles from the sample injection chamber through the cuvette in a single-file stream. Within the cuvette flow cell, laser light is focused on the sample core stream. Fluorescent molecules excited by the different laser wavelengths are detected by the optics and analyzed by the electronics.

Particles are then either transported to waste reservoirs via the waste aspirator, or sorted into a receptacle within the sort collection chamber. The following fluidics components are described in this section. For more information about fluidics, see Fluid Movement on page From there, the fluid travels to a second reservoir where it is held and pressurized until it flows into the cuvette flow cell.

This system eliminates pressure fluctuations by maintaining fluid levels and filtering out pump pulsation. As a result, the fluidics pressure does not vary with the level of fluid in the sheath containers.

Figure Plenum reservoirs.